Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells.

Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, Fang Q, Andrews DW
Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. Epub 2022 Apr 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
166761	Venus-BimEL(Noxa)-pEGFP-C1	To examine effect of swapping the BH3 region in full length BimEL with that of Noxa
166760	Venus-BimEL(Bik)-pEGFP-C1	To examine effect of swapping the BH3 region in full length BimEL with that of Bik
166759	Venus-BimEL(Bid)-pEGFP-C1	To examine effect of swapping the BH3 region in full length BimEL with that of Bid
166757	His6-TEV-mCerulean3-pBluescript	For purification of mCerulean3 fluorophore
166756	His6-TEV-Venus-pBluescript	For purification of Venus fluorophore
166755	mCerulean3-39aa-Venus-pEGFP-C3	For expression of mCerulean3 linked to Venus by 39 amino acid linker
166754	mCerulean3-31aa-Venus-pEGFP-C3	For expression of mCerulean3 linked to Venus by 31 amino acid linker
166753	mCerulean3-13aa-Venus-pEGFP-C3	For expression of mCerulean3 linked to Venus by 13 amino acid linker
166752	mCerulean3-Mcl-1-pLVX	Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.
166751	mCerulean3-Bcl-W-FUGW	Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.