Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells.
Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, Fang Q, Andrews DW
Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. Epub 2022 Apr 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166737 | Venus-Bik-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik |
| 166738 | Venus-Noxa-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa |
| 166739 | Venus-Puma-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma |
| 166743 | Venus-tBid-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166744 | Venus-Bik-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166745 | Venus-Noxa-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166746 | Venus-Puma-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166747 | Venus-tBid-2E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain |
| 166751 | mCerulean3-Bcl-W-FUGW | Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines. |
| 166752 | mCerulean3-Mcl-1-pLVX | Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines. |
| 166753 | mCerulean3-13aa-Venus-pEGFP-C3 | For expression of mCerulean3 linked to Venus by 13 amino acid linker |
| 166754 | mCerulean3-31aa-Venus-pEGFP-C3 | For expression of mCerulean3 linked to Venus by 31 amino acid linker |
| 166755 | mCerulean3-39aa-Venus-pEGFP-C3 | For expression of mCerulean3 linked to Venus by 39 amino acid linker |
| 166756 | His6-TEV-Venus-pBluescript | For purification of Venus fluorophore |
| 166757 | His6-TEV-mCerulean3-pBluescript | For purification of mCerulean3 fluorophore |
| 166759 | Venus-BimEL(Bid)-pEGFP-C1 | To examine effect of swapping the BH3 region in full length BimEL with that of Bid |
| 166760 | Venus-BimEL(Bik)-pEGFP-C1 | To examine effect of swapping the BH3 region in full length BimEL with that of Bik |
| 166761 | Venus-BimEL(Noxa)-pEGFP-C1 | To examine effect of swapping the BH3 region in full length BimEL with that of Noxa |