eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content.
Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. Epub 2023 May 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192227 | RPS15A sgRNA-1 lentiCRISPR v2 plasmid | lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR |
| 192228 | RPS15A sgRNA-2 lentiCRISPR v2 plasmid | lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR |
| 192229 | RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid | lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR |
| 192230 | RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid | lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR |
| 192231 | RPS4X sgRNA-1 lentiCRISPR v2 plasmid | lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR |
| 192232 | RPS4X sgRNA-2 lentiCRISPR v2 plasmid | lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR |
| 192233 | RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid | lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR |
| 192234 | RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid | lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR |
| 192235 | eIF3a- pMK289 (mAID-mClover-NeoR) plasmid | knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a |
| 192236 | eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid | knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a |
| 192237 | eIF3b- pMK289 (mAID-mClover-NeoR) plasmid | knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b |
| 192238 | eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid | knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b |
| 192239 | eIF3e- pMK289 (mAID-mClover-NeoR) plasmid | knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e |
| 192241 | eIF3f- pMK289 (mAID-mClover-NeoR) plasmid | knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f |
| 192242 | eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid | knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f |
| 192243 | eIF3k- pMK289 (mAID-mClover-NeoR) plasmid | knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k |
| 192244 | eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid | knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k |
| 192245 | pCDH-CMV-RPS15A-3xHA plasmid | overexpression vector of RPS15A-3XHA |
| 192246 | pCDH-CMV-RPS4X-3xHA plasmid | overexpression vector of RPS4X-3XHA |
| 192249 | eIF3b sgRNA-1 PX459 plasmid | expressing sgRNA-1 targeting eIF3b locus closed to stop coden |
| 192250 | eIF3b sgRNA-2 PX459 plasmid | expressing sgRNA-2 targeting eIF3b locus closed to stop coden |
| 192253 | eIF3f sgRNA-1 PX459 plasmid | expressing sgRNA-1 targeting eIF3f locus closed to stop coden |
| 192254 | eIF3f sgRNA-2 PX459 plasmid | expressing sgRNA-2 targeting eIF3f locus closed to stop coden |
| 192255 | eIF3k sgRNA-1 PX459 plasmid | expressing sgRNA-1 targeting eIF3k locus closed to stop coden |
| 192256 | eIF3k sgRNA-2 PX459 plasmid | expressing sgRNA-2 targeting eIF3k locus closed to stop coden |