The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection.

Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, Carette JE
Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
210490	plentiCRISPRv2-PURO-LYSET-gRNA2	CRISPR pooled KO of human LYSET
210489	plentiCRISPRv2-PURO-LYSET-gRNA1	CRISPR pooled KO of human LYSET
192006	pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag	Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter
192005	pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag	Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter
192004	pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro	Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter
192003	pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro	Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter
192002	pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro	Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter
192001	pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro	Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter
192000	pLenti-CMV-TMPRSS2-TwinStrep_Zeo	Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line
191999	pLenti_CMVTRE3G_LYSET-Isoform2_Flag	Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter