The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection.
Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, Carette JE
Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191998 | pLenti-CMV_ACE2-Flag_Hygro | Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line |
| 191999 | pLenti_CMVTRE3G_LYSET-Isoform2_Flag | Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter |
| 192000 | pLenti-CMV-TMPRSS2-TwinStrep_Zeo | Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line |
| 192001 | pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro | Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter |
| 192002 | pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro | Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter |
| 192003 | pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro | Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter |
| 192004 | pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro | Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter |
| 192005 | pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag | Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter |
| 192006 | pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag | Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter |
| 210489 | plentiCRISPRv2-PURO-LYSET-gRNA1 | CRISPR pooled KO of human LYSET |
| 210490 | plentiCRISPRv2-PURO-LYSET-gRNA2 | CRISPR pooled KO of human LYSET |