Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.
Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA
Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191535 | Human Lipid Droplet and Metabolism Library | Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism. |
| 202194 | pUC19/PLIN2-GFP | knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2 |
| 202195 | pUC19/PLIN2 GFP-P2A-BFP | knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2 |
| 202196 | pX330 PLIN2 | pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2 |