Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains.

Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
190087	pcDNA3_SARS-CoV-Spike[WIV1 RBD]	express chimeric SARS-CoV spike with the WIV-1 receptor binding domain
190086	pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]	express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain
190085	pcDNA3_SARS-CoV-Spike[PRD0038 RBD]	express chimeric SARS-CoV spike with the PRD0038 receptor binding domain
190084	pcDNA3_BtKY72-Spike	express BtKY72 spike
190083	pcDNA3_SARS-CoV-Spike[Khosta2 RBD]	express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain
190082	pcDNA3_SARS-CoV-Spike[Khosta1 RBD]	express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain
190081	pcDNA3_SARS-CoV-Spike[BtKY72 RBD]	express chimeric SARS-CoV spike with the BtKY72 receptor binding domain
190080	AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR	express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene
190079	AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR	express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
190078	AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR	express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene