Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains.
Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190076 | AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR | express human ACE2 with selectable markers iRFP670 and puromycin resistance gene |
| 190077 | AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR | express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene |
| 190078 | AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR | express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene |
| 190079 | AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR | express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene |
| 190080 | AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR | express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene |
| 190081 | pcDNA3_SARS-CoV-Spike[BtKY72 RBD] | express chimeric SARS-CoV spike with the BtKY72 receptor binding domain |
| 190082 | pcDNA3_SARS-CoV-Spike[Khosta1 RBD] | express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain |
| 190083 | pcDNA3_SARS-CoV-Spike[Khosta2 RBD] | express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain |
| 190084 | pcDNA3_BtKY72-Spike | express BtKY72 spike |
| 190085 | pcDNA3_SARS-CoV-Spike[PRD0038 RBD] | express chimeric SARS-CoV spike with the PRD0038 receptor binding domain |
| 190086 | pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD] | express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain |
| 190087 | pcDNA3_SARS-CoV-Spike[WIV1 RBD] | express chimeric SARS-CoV spike with the WIV-1 receptor binding domain |