pcDNA3_SARS-CoV-Spike[WIV1 RBD]
(Plasmid
#190087)
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Purposeexpress chimeric SARS-CoV spike with the WIV-1 receptor binding domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5278
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV S
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SpeciesSynthetic
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Insert Size (bp)3837
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Entrez GeneS (a.k.a. sars2, E2)
- Promoter CMV
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Tag
/ Fusion Protein
- Rhodopsin C9 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAATGGGCGGTAGGC
- 3′ sequencing primer CCTCGACTGTGCCTTCTA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byhttps://www.addgene.org/145031/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3_SARS-CoV-Spike[WIV1 RBD] was a gift from Kenneth Matreyek (Addgene plasmid # 190087 ; http://n2t.net/addgene:190087 ; RRID:Addgene_190087) -
For your References section:
Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains. Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA. PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. 10.1371/journal.pbio.3001738 PubMed 35895696
