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Circadian period is compensated for repressor protein turnover rates in single cells.

Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 10.1073/pnas.2404738121. Epub 2024 Aug 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
189980pDB2-hPER1-mScarlet-I-CD4-blaDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein
189981pDB2-hPER1-Luciferase-CD4-blaDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein
189982pDB2-hPER1-Halo-CD4-blaDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion protein
189984pDB2-hCRY2-mScarlet-I-CD4-blaDonor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion protein
189985pDB2-hCRY2-Luciferase-CD4-blaDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein
189986pDB2-hCRY2-Halo-CD4-blaDonor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein
189987pLentiCriprV2-sgRNA-PER1-#1Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982
189988pLentiCriprV2-sgRNA-PER1-#2Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982
189989pLentiCriprV2-sgRNA-CRY2-#1Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986
189991pL6-H2B-iRFP720-blaLentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance
189992pL6-H2B-iRFP720-puroLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Antibodies from Article