Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.

Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M
Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. PubMed Article

Plasmids from this article have been deposited by two labs. See below for all materials from each lab:

Sascha Martens

Michael Lazarou

Plasmids from Article

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ID	Plasmid	Purpose
199307	pBMN HA-NDP52	For expression of the mammalian NDP52 tagged with HA
199207	pBMN_HA-TBK1 (E696K)	Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)
199206	pBMN_HA-TBK1 (kinase dead - D135N)	Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)
199205	pBMN-HA-TBK1	Used for the expression of TBK1 (SMC Internal No. 1996)
198428	pGST2-GST-TEV-OPTN	Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)
198072	pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)	Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)
198037	pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K	Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)
198036	pCAGG-GST-TEV-mCherry-NAP1 (WT)	Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)
198035	pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)	Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)
198034	pCAGG-GST-TEV-NAP1 (WT)	Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)