Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition.
Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer ES
Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187116 | pAC8-DDIT4 | insect cell expression of DDIT4 |
| 187117 | pAC8-DDIT4_delta8 | insect cell expression of DDIT4-delta1-8 |
| 187118 | pAC8-Mcl1_deltaTM | insect cell expression of Mcl1-deltaTM |
| 187119 | pAC8-HUWE1-HECTonly | insect cell expression of HUWE1 HECT domain |
| 187120 | pDEST_FLAG-HUWE1 | mammalian cell expression of FLAG-HUWE1 |
| 187121 | pDARMO_CMVT_FLAG-HUWE1 | mammalian cell expression of FLAG-HUWE1 |
| 187122 | pDONR221-HUWE1_YH/GG | Entry clone |
| 187123 | pDEST_FLAG-HUWE1_YH/GG | mammalian cell expression of FLAG-HUWE1 Y355G/H356G |
| 187124 | pDONR221-HUWE1_delta3291-3298 | Entry clone |
| 187125 | pDEST_FLAG-HUWE1_delta3291-3298 | mammalian cell expression of FLAG-HUWE1 delta3291-3298 |
| 187126 | pDONR221-HUWE1_YH/GG_delta3291-3298 | Entry clone |
| 187127 | pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298 | mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298 |
| 187128 | pDONR221-HUWE1_I3969A/F3982A | Entry clone |
| 187129 | pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A | mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A |
| 187130 | pDONR221-HUWE1_H3962D | Entry clone |
| 187131 | pDARMO_CMVT_3xFLAG-HUWE1_H3962D | mammalian cell expression of 3xFLAG-HUWE1 H3962D |
| 187132 | pDONR221-HUWE1_delta3896-3951 | Entry clone |
| 187133 | pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951 | mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951 |
| 187134 | pDONR221-HUWE1_delta169-189 | Entry clone |
| 187135 | pDARMO_CMVT_3xFLAG-HUWE1_delta169-189 | mammalian cell expression of 3xFLAG-HUWE1 delta169-189 |
| 187136 | pDEST_FLAG-HUWE1_delta169-189 | mammalian cell expression of FLAG-HUWE1 delta169-189 |
| 187137 | pDONR221-HUWE1_H669Q | Entry clone |
| 187138 | pDARMO_CMVT_3xFLAG-HUWE1_H669Q | mammalian cell expression of 3xFLAG-HUWE1 H669Q |
| 187139 | pDONR221-HUWE1_F3194S | Entry clone |
| 187140 | pDEST_FLAG-HUWE1_F3194S | mammalian cell expression of FLAG-HUWE1 F3194S |
| 187141 | pDONR221-HUWE1_R4187C | Entry clone |
| 187142 | pDARMO_CMVT_3xFLAG-HUWE1_R4187C | mammalian cell expression of 3xFLAG-HUWE1 R4187C |
| 187143 | pDONR221-HUWE1_BH3-4E | Entry clone |
| 187144 | pDEST_FLAG-HUWE1_BH3-4E | mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E |
| 187145 | pDONR221-HUWE1_UBA-mut | Entry clone |
| 187146 | pDEST_FLAG-HUWE1_UBA-mut | mammalian cell expression of FLAG-HUWE1 M1328A/F1330A |
| 187147 | pDONR221-HUWE1_UIM-mut | Entry clone |
| 187148 | pDEST_FLAG-HUWE1_UIM-mut | mammalian cell expression of FLAG-HUWE1 A1376G/S1382A |
| 187149 | pDONR221-HUWE1_UBM-mut | Entry clone |
| 187150 | pDEST_FLAG-HUWE1_UBM-mut | mammalian cell expression of FLAG-HUWE1 L2976A/P2977A |
| 187151 | pDONR221-HUWE1_UBA/UIM-mut | Entry clone |
| 187152 | pDEST_FLAG-HUWE1_UBA/UIM-mut | mammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382A |
| 187153 | pDONR221-HUWE1_UBA/UIM/UBM-mut | Entry clone |
| 187154 | pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut | mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A |
| 187155 | pDARMO_HUWE1_3xFLAG | mammalian cell expression of HUWE1-3xFLAG |
| 187156 | pDEST_FLAG-HUWE1_C4341S | mammalian cell expression FLAG-HUWE C4341S |
| 190464 | pDONR221_HUWE1_isoform1 | Entry clone |