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Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions.

Xu X, Duan S, Hua X, Li Z, He R, Zhaang Z
Nat Commun. 2022 May 6;13(1):2514. doi: 10.1038/s41467-022-30298-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186930px459-H3.1 (Hist1h3g) sgRNAHist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal
186931px459-H3.3 (H3f3b) sgRNAH3f3b sgRNA used for genomic targeting at H3f3b C-terminal
186932pBlueScript II SK-SNAP donor for Hist1h3gGenomic targeting of SNAP tag at Hist1h3g C-terminal
186933pBlueScript II SK-SNAP donor for H3f3bGenomic targeting of SNAP tag at H3f3b C-terminal
186934px459-Pole3 KO sgRNAPole3 KO in mouse ES cells
186935px459-Pole4 KO sgRNAPole4 KO in mouse ES cells
186936px459-Mcm2-2A mutation sgRNAsgRNA used for genetic mutation at Y81 and Y90 of Mcm2
186937px459-Flag-Mcm2 targeting sgRNAGenomic targeting of Flag tag at Mcm2 N-terminal
186938px459-Hira KO sgRNAHira KO in mouse ES cells
186939px459-Daxx KO sgRNADaxx KO in mouse ES cells
186940px459-H3f3a KO sgRNAH3f3a KO in mouse ES cells
186941px459-H3f3b KO sgRNAH3f3b KO in mouse ES cells

Antibodies from Article