High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.
Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A
Nat Biotechnol. 2022 Aug 25:10.1038/s41587-022-01418-8. doi: 10.1038/s41587-022-01418-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186054 | pTR-337 pUC19-tNGFR-P2A-CTLA4 N | Knockin of truncated NGFR to target gene |
| 186055 | pTR-338 pUC19-tNGFR-P2A-LAG3 N | Knockin of truncated NGFR to target gene |
| 186056 | pTR-342 pUC19-tNGFR-P2A-CD28 N | Knockin of truncated NGFR to target gene |
| 186057 | pTR-449 tNGFR-P2A-CCR2 | Knockin of truncated NGFR to target gene |
| 186058 | pTR-373 tNGFR-P2A-IL7R | Knockin of truncated NGFR to target gene |
| 186059 | pTR-457 tNGFR-P2A-STAT1 | Knockin of truncated NGFR to target gene |
| 186060 | pTR-469 JUNB-tNGFR | Knockin of truncated NGFR to target gene |
| 186061 | pTR-471 IRF4-tNGFR | Knockin of truncated NGFR to target gene |
| 186062 | pTR-472 FOXO1-tNGFR | Knockin of truncated NGFR to target gene |
| 186063 | pTR-473 FOXP1-tNGFR | Knockin of truncated NGFR to target gene |
| 186064 | pTR-345 pUC19-tNGFR-P2A-IL2RB N | Knockin of truncated NGFR to target gene |
| 186065 | pTR-346 pUC19-tNGFR-P2A-IL2RA N | Knockin of truncated NGFR to target gene |
| 186066 | pTR-348 pUC19-tNGFR-P2A-ICOS N | Knockin of truncated NGFR to target gene |
| 186067 | pTR-340 pUC19-tNGFR-P2A-IL2RG N | Knockin of truncated NGFR to target gene |
| 186068 | pTR-374 tNGFR-P2A-B2M | Knockin of truncated NGFR to target gene |
| 186069 | pTR-375 tNGFR-P2A-ITGB7 | Knockin of truncated NGFR to target gene |
| 186070 | pTR-459 tNGFR-P2A-STAT6 | Knockin of truncated NGFR to target gene |
| 186071 | pTR-377 tNGFR-P2A-CD5 | Knockin of truncated NGFR to target gene |
| 186072 | pTR-378 tNGFR-P2A-CCR7 | Knockin of truncated NGFR to target gene |
| 186073 | pTR-379 tNGFR-P2A-CD48 | Knockin of truncated NGFR to target gene |
| 186074 | pTR-341 pUC19-tNGFR-P2A-PDCD1 N | Knockin of truncated NGFR to target gene |
| 186075 | pTR-460 tNGFR-P2A-STAT4 | Knockin of truncated NGFR to target gene |
| 186076 | pTR-458 tNGFR-P2A-STAT2 | Knockin of truncated NGFR to target gene |
| 186077 | pTR-339 pUC19-tNGFR-P2A-TIM3 N | Knockin of truncated NGFR to target gene |
| 186078 | pTR-450 tNGFR-P2A-CXCR4 | Knockin of truncated NGFR to target gene |
| 186080 | pTR-452 tNGFR-P2A-Lck | Knockin of truncated NGFR to target gene |
| 186081 | pTR-454 tNGFR-P2A-CARD11 | Knockin of truncated NGFR to target gene |
| 186082 | pTR-455 tNGFR-P2A-WAS | Knockin of truncated NGFR to target gene |
| 186083 | pTR-456 tNGFR-P2A-ITGB1 | Knockin of truncated NGFR to target gene |
| 186085 | pIG-712pUC19-tNGFR-P2A-ITCH | Knockin of truncated NGFR to target gene |
| 186086 | pIG-747pUC19-tNGFR-P2A-CD7 | Knockin of truncated NGFR to target gene |
| 186087 | pIG-735pUC19-tNGFR-P2A-PIK3CD | Knockin of truncated NGFR to target gene |
| 186088 | pIG-723pUC19-tNGFR-P2A-EVER1 | Knockin of truncated NGFR to target gene |
| 186089 | pIG-727pUC19-tNGFR-P2A-WASP | Knockin of truncated NGFR to target gene |
| 186090 | pIG-716pUC19-tNGFR-P2A-CD3G | Knockin of truncated NGFR to target gene |
| 186091 | pIG-714pUC19-tNGFR-P2A-SAP | Knockin of truncated NGFR to target gene |
| 186092 | pIG-738pUC19-tNGFR-P2A-Kindlin-3 | Knockin of truncated NGFR to target gene |
| 186093 | pIG-731pUC19-tNGFR-P2A-MAGT1 | Knockin of truncated NGFR to target gene |
| 186094 | pIG-728pUC19-tNGFR-P2A-WIP | Knockin of truncated NGFR to target gene |
| 186095 | pIG-729pUC19-tNGFR-P2A-Moesin | Knockin of truncated NGFR to target gene |
| 186096 | pIG-702pUC19-tNGFR-P2A-LRBA | Knockin of truncated NGFR to target gene |
| 186097 | pIG-708pUC19-tNGFR-P2A-DOCK8 | Knockin of truncated NGFR to target gene |
| 186098 | pIG-719pUC19-tNGFR-P2A-CD3Z | Knockin of truncated NGFR to target gene |
| 186099 | pIG-742pUC19-tNGFR-P2A-Caspase8 | Knockin of truncated NGFR to target gene |
| 186100 | pIG-718pUC19-tNGFR-P2A-CD3D | Knockin of truncated NGFR to target gene |
| 186101 | pIG-740pUC19-tNGFR-P2A-BCL10 | Knockin of truncated NGFR to target gene |
| 186102 | pIG-704pUC19-tNGFR-P2A-STAT3 | Knockin of truncated NGFR to target gene |
| 186103 | pIG-705pUC19-tNGFR-P2A-STAT5b | Knockin of truncated NGFR to target gene |
| 186104 | pIG-736pUC19-tNGFR-P2A-CD40L | Knockin of truncated NGFR to target gene |
| 186105 | pIG-724pUC19-tNGFR-P2A-EVER2 | Knockin of truncated NGFR to target gene |
| 186106 | pIG-748pUC19-tNGFR-P2A-AP3B1 | Knockin of truncated NGFR to target gene |
| 186107 | pIG-734pUC19-tNGFR-P2A-PIK3R1 | Knockin of truncated NGFR to target gene |
| 186108 | pIG-710pUC19-tNGFR-P2A-IL10R1 | Knockin of truncated NGFR to target gene |
| 186109 | pIG-713pUC19-tNGFR-P2A-CD45 | Knockin of truncated NGFR to target gene |
| 186110 | pIG-730pUC19-tNGFR-P2A-Coronin1A | Knockin of truncated NGFR to target gene |
| 186111 | pIG-717pUC19-tNGFR-P2A-CD3E | Knockin of truncated NGFR to target gene |
| 186112 | pIG-703pUC19-tNGFR-P2A-BACH2 | Knockin of truncated NGFR to target gene |
| 186113 | pIG-737pUC19-tNGFR-P2A-CD18 | Knockin of truncated NGFR to target gene |
| 186114 | pIG-701pUC19-tNGFR-P2A-FOXP3 | Knockin of truncated NGFR to target gene |
| 186115 | pIG-745pUC19-tNGFR-P2A-CD4 | Knockin of truncated NGFR to target gene |
| 186116 | pIG-721pUC19-tNGFR-P2A-RhoH | Knockin of truncated NGFR to target gene |
| 186117 | pIG-709pUC19-tNGFR-P2A-IL10 | Knockin of truncated NGFR to target gene |
| 186118 | pIG-744pUC19-tNGFR-P2A-CD2 | Knockin of truncated NGFR to target gene |
| 186119 | pIG-732pUC19-tNGFR-P2A-STIM1 | Knockin of truncated NGFR to target gene |
| 186120 | pIG-720pUC19-tNGFR-P2A-ZAP70 | Knockin of truncated NGFR to target gene |
| 186121 | pIG-746pUC19-tNGFR-P2A-CD5 | Knockin of truncated NGFR to target gene |
| 186122 | pIG-707pUC19-tNGFR-P2A-TTC37 | Knockin of truncated NGFR to target gene |
| 186123 | pIG-648 CTLA4int1-CTLA4-GFP-SV40 | CTLA4 gene knockin |
| 186124 | pIG-719 CTLA4-GFP R70W | CTLA4 gene knockin with mutation |
| 186125 | pIG-720 CTLA4-GFP R75W | CTLA4 gene knockin with mutation |
| 186126 | pIG-721 CTLA4-GFP T124P | CTLA4 gene knockin with mutation |
| 186127 | pIG-641 JE-TRAC-BCMA(BB)28z-P2A | BCMA-CAR DNA production |
| 186128 | pTR-177 pUC19-CLTA-N-mCherry | knockin of mCherry to CLTA target |