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Androgen receptor and MYC equilibration centralizes on developmental super-enhancer.

Guo H, Wu Y, Nouri M, Spisak S, Russo JW, Sowalsky AG, Pomerantz MM, Wei Z, Korthauer K, Seo JH, Wang L, Arai S, Freedman ML, He HH, Chen S, Balk SP
Nat Commun. 2021 Dec 15;12(1):7308. doi: 10.1038/s41467-021-27077-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171218LV-AR-AAFFLenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.
171219LV-AR-KKKAAALenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
171220LV-AR-C563A-C596ALenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
171221LV-AR-R599A-N600A Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.
171222LV-AR-C619YLenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
213819LV-ARLenti-viral expression of human androgen receptor wild-type (WT).

Antibodies from Article