A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag.
Yim WW, Yamamoto H, Mizushima N
Elife. 2022 Aug 8;11. pii: 78923. doi: 10.7554/eLife.78923.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184899 | pMRX-IP-HaloTag7-LC3 | Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux |
| 184900 | pMRX-No-HaloTag7-LC3 | Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux |
| 184901 | pMRX-No-HaloTag7-mGFP-LC3 | Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux |
| 184902 | pMRX-No-HaloTag7-mGFP-LC3-mRFP | Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux |
| 184903 | pMRX-IB-HaloTag7-mGFP | Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux |
| 184904 | pMRX-IB-HaloTag7-mGFP-KDEL | Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity |
| 184905 | pMRX-IB-pSu9-HaloTag7-mGFP | Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity |