Improved CRISPR-Cas12a-assisted one-pot DNA editing method enables seamless DNA editing.
Wang L, Wang H, Liu H, Zhao Q, Liu B, Wang L, Zhang J, Zhu J, Bao R, Luo Y
Biotechnol Bioeng. 2019 Jun;116(6):1463-1474. doi: 10.1002/bit.26938. Epub 2019 Feb 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183636 | PET28-FnCas12a-EP16 | PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA. |