SyNPL: Synthetic notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo.
Malaguti M, Migueles RP, Annoh J, Sadurska D, Blin G, Lowell S
Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183603 | pPyCAG-EGFP-TM-IRES-Pac | Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance. |
| 183604 | pPyPGK-EGFP-TM-IRES-Pac | Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance. |
| 183605 | pPyCAG-HA-EGFP-Myc-TM-IRES-Pac | Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance. |
| 183606 | pPyPGK-HA-EGFP-Myc-TM-IRES-Pac | Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance. |
| 183607 | pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble | Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance. |
| 183608 | pPyCAG-tagBFP-3xNLS-IRES-Hph | Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance. |
| 183609 | pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 | Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS. |
| 183610 | attB53-Pac-attB53 | RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609). |
| 183611 | attB53-Pac-TRE-mCherry-attB53 | RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609). |
| 183612 | attB53-Pac-tetO-mCherry-attB53 | RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609). |
| 183613 | attB53-Pac-TRE-3xFlag-Neurog1-attB53 | RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609). |
| 183614 | attB53_SNCB+_attB53 | RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand. |
| 183615 | attB53_SNCB-_attB53 | RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense. |