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"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification.

Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, Pruner MT, Dam Ferdinez ML, Wickersham IR
Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 10.1073/pnas.2023481120. Epub 2023 Feb 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115234pLV-CAG-FLEX-BFP2-(mCherry)’Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it
115235pLV-CAG-F14F15S-BFP2-(mCherry)'Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it
115236pLV-U-TVA950Lentiviral transfer plasmid for expression of TVA950
122050pRVdG-4FLPoGenome plasmid for deletion-mutant rabies virus encoding Flpo recombinase
174377pB-CAG-TEVp-IRES-mCherryFor making TEVP cell line with hypBase
174379pAAV-TREtight-H2b-emiRFP670-P2A-TEVPAAV virus for expressing TEVP and H2b-emiRFP670
174380pRV∆G-NPEST-4CreRabies vector for expressing Cre recombinase and N gene with PEST domain
174381pRV∆G-N*PEST-4CreRabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Antibodies from Article