Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not require an intact p53 pathway.
Stransky LA, Vigeant SM, Huang B, West D, Denize T, Walton E, Signoretti S, Kaelin WG Jr
Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120403119. doi: 10.1073/pnas.2120403119. Epub 2022 Mar 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183112 | pLenti_EF1a_Flag_TP53_WT | Expresses flag-tagged p53 in mammalian cells |
| 183113 | pLenti_EF1a_Flag_TP53_P278A | Expresses flag-tagged p53 with P278A mutation in mammalian cells |
| 183114 | pLenti_EF1a_Flag_TP53_R248W | Expresses flag-tagged p53 with R248W mutation in mammalian cells |
| 183115 | pLenti_EF1a_Flag_TP53_R248W+P278A | Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells |
| 183116 | pLenti_EF1a_EV | Empty vector control for pLenti_EF1a constructs |
| 183117 | pLenti_EF1a_Flag_EGFP | Expresses flag-tagged EGFP in mammalian cells |
| 183118 | pLentiGuide_GFP_NT135 | pLentiGuide modified to express GFP and a non-targeting sgRNA |
| 183119 | pLentiGuide_GFP_sgEPAS1#1 | pLentiGuide modified to express GFP and an sgRNa targeting EPAS1 |
| 183120 | pLentiGuide_GFP_sgEPAS1#2 | pLentiGuide modified to express GFP and an sgRNA targeting EPAS1 |
| 183121 | pLentiGuide_mCherry_NT135 | pLentiGuide modified to express mCherry and a non-targeting sgRNA |
| 183122 | pLentiGuide_mCherry_sgEPAS1#1 | pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1 |
| 183123 | pLentiGuide_mCherry_sgEPAS1#2 | pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1 |
| 183180 | pLentiCRISPR_zeo_sgTP53 | pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53 |
| 183181 | pLentiCRISPR_zeo_sgNT135 | pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA |
| 187818 | pLentiCRISPR_Puro_sgAAVS1 | pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1 |
| 187819 | pLentiCRISPR_Puro_sgTP53 | pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53 |