Rapid, Efficient, and Cost-Effective Gene Editing of Enterococcus faecium with CRISPR-Cas12a.

Chua MJ, Collins J
Microbiol Spectr. 2022 Feb 2:e0242721. doi: 10.1128/spectrum.02427-21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
182746	pUCsRNAP	Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
182739	pJC005.gent	CRISPR-Cas12a genome editing of E. faecium
182738	pJC005.em	CRISPR-Cas12a genome editing of E. faecium