A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast.
Shively CA, Dong F, Mitra RD
ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
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Article
A subset of these plasmids is included in the CEAS (C-SWAT for Essential Allele Strains) Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182509 | C-SWAT 6FLAG | C-SWAT compatible vector for C-terminal tagging with 6 FLAG |
| 182510 | C-SWAT GFP | C-SWAT compatible vector for C-terminal tagging with GFP |
| 182511 | C-SWAT GFP DAmP | C-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette. |
| 182512 | C-SWAT GFP CL1 | C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown. |
| 182513 | C-SWAT GFP CL1 DAmP | C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette. |
| 182514 | C-SWAT AID*-GFP osTIR1 | C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration. |
| 182515 | C-SWAT AID*-6FLAG osTIR1 | C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome. |
| 182516 | C-SWAT IAA17-6FLAG | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration. |
| 182517 | C-SWAT IAA17-3HA | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration. |
| 182518 | C-SWAT IAA17-3MYC | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration. |
| 182519 | Gal1/10p CAS9 Gal1/10p I-SceI | Galactose-Induced Expression vector for Cas9 and I-SceI. |
| 182520 | SIR4 gRNA HIS | Expression vector for gRNA directed against SIR4 ORF. |
| 182521 | CAN1 gRNA HIS | Expression vector for gRNA directed against CAN1 ORF. |
| 182522 | C-SWAT IAA17-6FLAG | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration. |
| 182523 | C-SWAT SMASh | C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL). |
| 182524 | C-SWAT 3MYC SMASh | C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL). |
| 182525 | C-SWAT 3MYC SMASh | C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL). |
| 182526 | NAT-TET-OFF -MET - 3xHA | Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging. |
| 182527 | NAT-TET-OFF -LEU- 4xFLAG | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193314 | NAT-TET-OFF – ubiquitin – LEU – 4MYC | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193315 | HYG-TET-OFF – ubiquitin – LEU – 4FLAG | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193316 | HYG-TET-OFF – ubiquitin – LEU – 4MYC | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193317 | KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193318 | KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC | Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine. |
| 193319 | C-SWAT IAA17 | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration. |
| 193320 | C-SWAT AID*-6MYC osTIR1 | C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome. |
| 193321 | C-SWAT Compatible pAIDA2(6FLAG) | C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag. |
| 193322 | C-SWAT IAA17-6FLAG (no NLS) | C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags.. |