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Ferroptosis regulation by the NGLY1/NFE2L1 pathway.

Forcina GC, Pope L, Murray M, Dong W, Abu-Remaileh M, Bertozzi CR, Dixon SJ
Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118646119. doi: 10.1073/pnas.2118646119. Epub 2022 Mar 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
181916pLenti-CMV-NGLY1-PuroLentiviral plasmid that directs the expression of NGLY1 in mammalian cells.
181917pTwist-SFFV-NFE2L1-PuroLentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.
181918pTwist-SFFV-NFE2L18ND-PuroLentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.
181919pLenti-CMV-NFE2L2-PuroLentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Antibodies from Article