Highly efficient generation of isogenic pluripotent stem cell models using prime editing.
Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F
Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180014 | AAVS1-SA-neo-CAGGS-PE2-2A-GFP | Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus |
| 180015 | pET30a(+)-PE2 | Expressing PE2 protein in E.coli for protein purification |
| 180016 | pU6-pegRNA-SNCA-A30P | Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells |
| 180017 | pU6-pegRNA-HEK3-CTTins-px330-scaffold | Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330. |
| 180018 | pBPK1520-SNCA-A30P-ng | Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach |
| 180019 | pBPK1520-HEK3-CTTins-ng | Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach |
| 180432 | pU6-pegRNA-LRRK2-G2019S-3a | Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells |
| 180433 | pU6-pegRNA-LRRK2-G2019S-3b | Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells |
| 180434 | pU6-pegRNA-LRRK2-G2019S-3c | Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells |
| 180435 | pU6-pegRNA-LRRK2-G2019S-3d | Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells |
| 180436 | BPK1520-LRRK2-G2019S-ng | Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach |
| 180437 | Px330-EGFP-LRRK2-CRISPR/Cas9 | Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells |
| 181738 | pU6-pegRNA-SNCA-A53T | Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells |
| 181739 | pBPK1520-SNCA-A53T-ng | Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach |