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SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Miyata H, Oura S, Morohoshi A, Shimada K, Mashiko D, Oyama Y, Kaneda Y, Matsumura T, Abbasi F, Ikawa M
Proc Natl Acad Sci U S A. 2021 Aug 31;118(35). pii: 2106673118. doi: 10.1073/pnas.2106673118. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
179129pCAG-mSpata33-PAExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
179130pCAG-mSpata33 (P79A)-PAExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
179131pCAG-mSpata33 (I81A)-PAExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
179132pCAG-mSpata33 (I83A)-PAExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
179133pCAG-hSPATA33-8xHIS-1D4Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.
179134pCAG-hPPP3CA-FLAGExpression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
179135pCAG-hPPP3CB-FLAGExpression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
179136pCAG-hPPP3R1Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Antibodies from Article