T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition.
Hui E, Cheung J, Zhu J, Su X, Taylor MJ, Wallweber HA, Sasmal DK, Huang J, Kim JM, Mellman I, Vale RD
Science. 2017 Mar 31;355(6332):1428-1433. doi: 10.1126/science.aaf1292. Epub 2017 Mar 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177896 | pET28a(His10-PD-1cyto) | E coli expression of His10 tag fused to human PD-1 Intracellular Domain |
| 177897 | pFastBac (GST-SNAP-Shp2) | Baculo expression of GST tag fused to SNAP tag and human Shp2 |
| 177899 | pFastBac(His10-Shp2) | Baculo expression of His10 tag fused to human Shp2 |
| 177900 | pET28a(cd226) | Bacterial expression of his10-tagged human cd226 Intracellular Domain |
| 177901 | pET28a(cd96) | Bacterial expression of his10-tagged human cd96 Intracellular Domain |
| 177902 | pET28a(TIGIT) | Bacterial expression of his10-tagged human TIGIT Intracellular Domain |
| 177903 | pET28a(ICOS) | Bacterial expression of his10-tagged human ICOS Intracellular Domain |
| 177904 | pET28a(DAP10) | Bacterial expression of his10-tagged human DAP10 Intracellular Domain |
| 177905 | pET28a(CTLA4) | Bacterial expression of his10-tagged human CTLA4 Intracellular Domain |
| 177907 | pGEX6P2(GST-SNAP-Shp2tSH2) | Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains |