In vitro membrane reconstitution of the T-cell receptor proximal signaling network.
Hui E, Vale RD
Nat Struct Mol Biol. 2014 Feb;21(2):133-42. doi: 10.1038/nsmb.2762. Epub 2014 Jan 26.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
177887 | pFastBacHT (His10-4xFRB-SNAP-Lck G2A) | Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A |
177889 | pFastBac (GST-preSci site-CD45) | Baculo expression of GST tag fused to human CD45 ICD |
177891 | pFastBac(His10-Lckg2a) | Baculo expression of His10 tag fused to human LckG2A |
177893 | pFastBac(His10-Lckg2a,Y394F) | Baculo expression of His10 tag fused to human LckG2A |
177894 | pFastBac(His10-Lckg2a,Y394F,Y505F) | Baculo expression of His10 tag fused to human LckG2A |
177895 | pFastBac(His10-SNAP-Csk) | Baculo expression of His10 tag fused to SNAPtag and human Csk |