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In vitro membrane reconstitution of the T-cell receptor proximal signaling network.

Hui E, Vale RD
Nat Struct Mol Biol. 2014 Feb;21(2):133-42. doi: 10.1038/nsmb.2762. Epub 2014 Jan 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
177887pFastBacHT (His10-4xFRB-SNAP-Lck G2A)Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A
177889pFastBac (GST-preSci site-CD45)Baculo expression of GST tag fused to human CD45 ICD
177891pFastBac(His10-Lckg2a)Baculo expression of His10 tag fused to human LckG2A
177893pFastBac(His10-Lckg2a,Y394F)Baculo expression of His10 tag fused to human LckG2A
177894pFastBac(His10-Lckg2a,Y394F,Y505F)Baculo expression of His10 tag fused to human LckG2A
177895pFastBac(His10-SNAP-Csk)Baculo expression of His10 tag fused to SNAPtag and human Csk

Antibodies from Article