PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.
Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma Y, Wang H, Bennett EJ, Fu G, Hui E
J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 10.1083/jcb.201905085.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180790 | pHR-PD-1 (Y223F, Y248F)-mGFP | Expression of human PD-1 (Y223F, Y248F) fused to mGFP |
| 180791 | pHR-BTLA-mGFP | Expression of human BTLA fused to mGFP |
| 180792 | pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP | Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mGFP |
| 180794 | pHR-PD-1ecto&tmd-BTLAicd-mGFP | Expression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mGFP |
| 180795 | pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP | Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mGFP |
| 180798 | pMIG II-BTLA-mGFP | Expression of mouse BTLA fused to mGFP |
| 180799 | pMIG II SIN-SV40-mCherry-SHP1 | Expression of mouse SHP1 fused to N-terminal mCherry |
| 180800 | pMIG II SIN-SV40-mCherry-SHP2 | Expression of mouse SHP2 fused to N-terminal mCherry |
| 180805 | pET28-His10-BTLAicd (AA190-289)-TwinStrep | Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep |
| 180806 | pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep | Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep |
| 180807 | pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep | Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep |
| 180808 | pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep | Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep |
| 180809 | pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep | Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep |
| 180811 | pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep | Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep |
| 180812 | pET28a-His10-SHP1_PTPase (243-541aa) | Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa) |
| 180814 | pGEX-6p-2-GST-PD-1icd (AA194-288) | Expression of GST fused human PD-1 intracellular domain (AA194-288) |
| 180815 | pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F) | Expression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F) |