David Andrews Lab: David Andrews Lab plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
177407	mCherry-7aa-cb5-pEGFP-C1	To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
177410	Venus-BclXL-ActA-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177411	mCerulean3-BclXL-ActA-s2193	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177412	mCerulean3-BclXL-ActA-pEGFP-C1	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177413	mCerulean3-BclXL-Cb5-s2193	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
177414	mCerulean3-BclXL-Cb5-pEGFP-C1	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
177415	Venus-BclXL-Cb5-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
177418	mCerulean3-Bcl2-ActA-pEGFP-C1	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177419	Venus-Bcl2-ActA-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177420	mCerulean3-Bcl2-ActA-s2193	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
177421	mCerulean3-Bcl2-cb5-pEGFP-C1	Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
177422	Venus-Bcl2-cb5-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
177423	mCerulean3-Bcl2-cb5-s2193	To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
177425	mCerulean3-Bik-s2193	To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
177426	Venus-Bik-L61G-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
177427	Venus-Bik-GG153AA-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
177428	Venus-Bik-G154STOP-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
177429	Venus-Bik-DD-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
177430	Venus-Bik-AA-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
177431	Venus-Bik-cb5-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
177432	Venus-Bik-delMBR-pEGFP-C1	To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
177433	mCherry-ActA-IRES-puromycin-pLVx-EF1a	To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
177434	mCherry-Cb5-IRES-puromycin-pLVx-EF1a	To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
177435	mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a	To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.