Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation.
Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, Liu Z, Mancini MA, Li W
Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177120 | plenti-ef1a-dCasRx-FTO-HA-T2A-BSD | dCasRx mediated RNA tethering of FTO |
| 177121 | pTXBI-mclover-YTHDC1-FL | Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification |
| 177122 | pTXBI-mclover-YTH-IDR2 | Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification |
| 177123 | pTXBI-mClover-YTH domain | Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification |
| 177124 | pHR-YTHDC1-FL-mCherry-Cry2 | Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay |
| 177125 | pHR-IDR1-mCherry-Cry2 | Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay |
| 177126 | pHR-YTH-mCherry-Cry2 | Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay |
| 177130 | pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant | Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1 |