Yannick Doyon Lab: S-phase specific probe
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176932 | AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108) | Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus |
| 191387 | hSLBP_mNG_FS_Donor | Donor for C-terminal tagging of endogenous hSLBP |
| 191388 | SpCas9_hGEM(1-110)_hSLBP(56-108) | SpCas9 fused with geminin and SLBP degrons to improve HDR ratio |
| 191389 | hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100) | Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus |
| 191390 | hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy- | Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus |
| 191391 | hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120) | Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus |
| 191442 | eSpCas9(1.1)_NoFlag_hROSA26_Left | Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus |
| 191527 | AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS | Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus |
| 191528 | eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA | Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain |
| 191529 | AAVS1_Puro_H2BK_mNG_hSLBP(18-108) | Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus |