Targeting oncoproteins with a positive selection assay for protein degraders.

Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
176298	pLX304-DCK*-IRES-Td	Expresses DCK* and TdTomato from the same transcript
176297	pLX304-DCK*-IRES-GFP	Expresses DCK* and GFP from the same transcript
176296	pLX304-IRES-Td	pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites
176291	pLX304-GW-DCK*-IRES-GFP	Gateway vector for use in generating POI-DCK* fusion reporter
176289	pLX304-DCK*-GW-IRES-GFP	Gateway vector for use in generating DCK*-POI fusion reporter
176288	pLX304-IRES-GFP	pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites