Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission.
Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K
Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175180 | pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt | Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857 |
| 175181 | pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 175182 | pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt | Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ |
| 175184 | pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt | Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI |
| 175186 | pRSET iGluSnFR3 v857 | Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria. |
| 175187 | pRSET iGluSnFR3 v82 | Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria. |
| 178329 | pAAV.hSyn.iGluSnFR3.v857.PDGFR | Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857 |
| 178330 | pAAV.hSyn.iGluSnFR3.v857.SGZ | Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ |
| 178331 | pAAV.hSyn.iGluSnFR3.v857.GPI | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 178333 | pAAV.CAG.iGluSnFR3.v857.PDGFR | Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857 |
| 178334 | pAAV.CAG.iGluSnFR3.v857.SGZ | Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ |
| 178335 | pAAV.CAG.iGluSnFR3.v857.GPI | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 178336 | pAAV.GFAP.iGluSnFR3.v857.PDGFR | Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857 |
| 178337 | pAAV.GFAP.iGluSnFR3.v857.SGZ | Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ |
| 178338 | pAAV.GFAP.iGluSnFR3.v857.GPI | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 196218 | pAAV.CAG.FLEX.iGluSnFR3.v857.GPI | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 196219 | pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR | Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR |
| 196223 | pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI | Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI |
| 196224 | pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ | Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ |
| 196226 | pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR | Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR |
| 196227 | pAAV.hSyn.iGluSnFR3.v857.IgK-NGR | Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR |