Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition.
Maier NK, Ma J, Lampson MA, Cheeseman IM
Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174704 | 2xmNeonGreen-hMeikin | Stable expression of human Meikin |
| 174705 | TetO::EGFP-hMeikin | Doxycycline inducible expression of human Meikin |
| 174706 | TetO::EGFP-C-Meikin | Doxycycline inducible expression of human C-Meikin Separase cleavage fragment |
| 174707 | TetO::EGFP-hMeikin(8A mut) | Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding |
| 174708 | 3xEGFP-mMeikin | Mammalian expression of mouse Meikin |
| 174710 | 3xEGFP-mMeikin (E156R, R159E) | Mammalian expression of mouse Meikin with Separase-resistant mutations |
| 174712 | 3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434) | Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted |
| 174713 | 3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434) | Mammalian expression of mouse Meikin with inserted C-terminal Separase cleavage site inactivated |
| 174714 | H2B-mScarlet- hMeikin(1-332)-mNeonGreen | Stable expression of fluorescent Separase cleavage sensor with human Meikin |
| 174715 | H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen | Stable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutations |
| 174716 | H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen | Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin |
| 174717 | H2B-mScarlet- hRec8(297-506)-mNeonGreen | Stable expression of fluorescent Separase cleavage sensor with human Rec8 |