Direct observation of aggregate-triggered selective autophagy in human cells.

Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
174650	pBA-mKeima-PIM	Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
174651	pLVX-IRES-hygro-EGFP-DFCP1	Vector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.
174652	pLVX-IRES-hygro-EGFP-Stx17	Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.