The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway.
Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi G, Jones DR, Kimmelman AC, Pacold ME
Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 10.1038/s41586-021-03865-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174128 | pLVU-coHPDL WT | Expresses wild type codon-optimized HPDL in mammalian cells |
| 174129 | pLenti6.2-coHPDL WT 3xFlag-V5 | Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells |
| 174130 | pLenti6.2-coHPDL H258A 3xFlag-V5 | Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells |
| 174131 | pLenti6.2-coHPDL H163A H258A 3xFLAG-V5 | Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells |
| 174165 | TLCV2-sgHPDL #3 | knock out HPDL in human cell lines |
| 174166 | TLCV2-sgHPDL #9 | knock out HPDL in human cell lines |