Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling.
Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW
Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173932 | pET41sIII_GST-ALC1 | Bacterial expression of ALC1 |
| 173933 | pET28_6xHis-PARP1delZn1 | Bacterial expression of PARP1 lacking Zn1 domain |
| 173934 | pET28_6xHis-PARP1delZn3-L713F | Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation |
| 173935 | pET28_6xHis-PARP1delZn2 | Bacterial expression of PARP1 lacking Zn2 domain |
| 173936 | pET28_6xHis-PARP1delZn3 | Bacterial expression of PARP1 lacking Zn3 domain |
| 173937 | pET28_6xHis-PARP1delZn1-L713F | Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation |
| 173938 | pET28_6xHis-PARP1delBRCT | Bacterial expression of PARP1 lacking BRCT domain |
| 173939 | pET28_6xHis-PARP1delZn2delBRCT | Bacterial expression of PARP1 lacking Zn2 and BRCT domains |
| 173940 | pET28_6xHis-PARP1delWGR | Bacterial expression of PARP1 lacking WGR domain |
| 173941 | pET28_6xHis-PARP1delWGR-L713F | Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation |
| 173942 | pET28_6xHis-PARP1delCAT | Bacterial expression of PARP1 lacking CAT domain |
| 173943 | pET28_6xHis-PARP1-CAT | Bacterial expression of isolated PARP1 CAT domain |
| 173944 | pET28_6xHis-PARP1-CAT-L713F | Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation |
| 173945 | pET28_6xHis-PARP1-E988K | Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1) |
| 174792 | pET28_6xHis-PARP1-C125G | Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break) |
| 174793 | pET28_6xHis-PARP1-W318R | Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding) |
| 174794 | pET28_6xHis-PARP1-L713F | Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain) |
| 174795 | pET28_6xHis-PARP1-A762V | Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism) |
| 174796 | pET28_6xHis-PARP1-A774L | Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain) |
| 174797 | pET28_6xHis-PARP1-A870L | Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain) |
| 174798 | pET28_6xHis-PARP1-G871L | Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain) |
| 174799 | pET28_6xHis-PARP1-K893I | Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding) |
| 174800 | pET28_6xHis-PARP1-G972R | Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall |
| 174801 | pET28_6xHis-PARP1-Y986H | Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall |
| 174802 | pET28_6xHis-PARP1-Y986S | Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers |
| 174804 | pET28_6xHis-PARP1deltaART | Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain |
| 174805 | pET28_6xHis-PARP1-Zn2 | Bacterial expression of isolated PARP1 Zn2 domain |
| 174806 | pET28_6xHis-PARP1-Zn3 | Bacterial expression of isolated PARP1 Zn3 domain |
| 174807 | pET28_6xHis-PARP1-BRCT | Bacterial expression of isolated PARP1 BRCT domain |
| 174808 | pET28_6xHis-PARP1-WGR | Bacterial expression of isolated PARP1 WGR domain |
| 174809 | pET28_6xHis-PARP1-HD | Bacterial expression of isolated PARP1 HD subdomain |
| 174810 | pET28_6xHis-PARP1-ART | Bacterial expression of isolated PARP1 ART subdomain |
| 174811 | pET28_6xHis-PARP1-CATdeltaHD | Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain |
| 174813 | pET41sIII_GST-ALC1-K77R | Bacterial expression of inactive ALC1 (K77R disrupts ATP binding) |
| 174814 | pET41sIII_GST-ALC1-E175Q | Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding) |
| 175949 | pET28_6xHis-GAL4-PARP1-CAT-L713F | Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation |