CRL4(AMBRA1) is a master regulator of D-type cyclins.
Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, Jones DR, Busino L, Pagano M
Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y. Epub 2021 Apr 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172603 | pBABE-MCS(extended)-Puro (empty vector) | Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells. |
| 172604 | pcDNA3.1-2xFLAG-2xSTREP (empty vector) | Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells |
| 172605 | pcDNA3.1-AMBRA1-3xFLAG | Expresses 3xFLAG-tagged AMBRA1 in mammalian cells |
| 172606 | px459-AMBRA1 #3 | Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro |
| 172607 | px458-AMBRA1 #3 | Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP |
| 172608 | px458-AMBRA1 (optimized for N-terminal knock-in) | Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1. |
| 172609 | pUC57-2xFLAG-mAID-AMBRA1 HDR donor template | Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene |
| 172610 | pTRIPZ-TIR1-HA | Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells |
| 172611 | pMSCV-FLAG-2xSTREP-CDKN1B-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells |
| 172612 | pcDNA3.1-HA-CDK4 | Expresses HA-tagged CDK4 in mammalian cells |
| 172613 | pcDNA3.1-HA-CDK2 | Expresses HA-tagged CDK2 in mammalian cells |
| 172614 | pcDNA3.1-2xFLAG-2xSTREP-CDK6 | Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells |
| 172615 | pcDNA3.1-2xFLAG-2xSTREP-CDK4 | Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells |
| 172616 | pcDNA3.1-2xFLAG-2xSTREP-CDK2 | Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells |
| 172617 | pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells |
| 172618 | pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells |
| 172619 | pMSCV-FLAG-2xSTREP-CCND3-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells |
| 172620 | pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells |
| 172621 | pcDNA3.1-HA-CCND3 | Expresses HA-tagged cyclin D3 in mammalian cells |
| 172622 | pBABE-2xFLAG-2xSTREP-CCND3-Puro | Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells |
| 172623 | pcDNA3.1-2xFLAG-2xSTREP-CCND3 | Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells |
| 172624 | pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells |
| 172625 | pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells |
| 172626 | pMSCV-FLAG-2xSTREP-CCND2-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells |
| 172627 | pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells |
| 172628 | pBABE-2xFLAG-2xSTREP-CCND2-Puro | Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells |
| 172629 | pcDNA3.1-2xFLAG-2xSTREP-CCND2 | Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells |
| 172630 | px458-CCND1 | Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP |
| 172631 | pcDNA3.1-CCND1(T286A) | Expresses untagged cyclin D1(T286A) in mammalian cells |
| 172632 | pcDNA3.1-CCND1 | Expresses untagged cyclin D1 in mammalian cells |
| 172633 | pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro | Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells |
| 172634 | pBABE-2xFLAG-2xSTREP-CCND1-Puro | Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells |
| 172635 | pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells |
| 172636 | pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells |
| 172637 | pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells |
| 172638 | pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells |
| 172639 | pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells |
| 172640 | pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry | Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells |
| 172641 | pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells |
| 172642 | pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells |
| 172643 | pMSCV-FLAG-2xSTREP-CCND1-Puro | Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells |
| 172644 | pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S) | Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells |
| 172645 | pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I) | Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells |
| 172646 | pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L) | Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells |
| 172647 | pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A) | Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells |
| 172648 | pcDNA3.1-2xFLAG-2xSTREP-CCND1 | Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells |
| 172649 | pcDNA3.1-HA-CCND1 | Expresses HA-tagged cyclin D1 in mammalian cells |