DogCatcher allows loop-friendly protein-protein ligation.
Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M
Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171772 | pDEST14-DogCatcher | Expresses DogCatcher protein in bacterial cytoplasm |
| 171773 | pET28-AviTag-DogTag-MBP | Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA. |
| 171775 | pET28a-HaloTag7SS-DogTag | Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation |
| 171776 | pET28-sfGFP-SpyTag003 Loop A | Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23 |
| 171777 | pET28-sfGFP-SpyTag003 Loop B | Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103 |
| 171778 | pET28-sfGFP-SpyTag003 Loop C | Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174 |
| 171779 | pET28-sfGFP-DogTag Loop A | Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23 |
| 171780 | pET28-sfGFP-DogTag Loop B | Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103 |
| 171781 | pET28-sfGFP-DogTag Loop C | Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174 |
| 171782 | pET28-MBP-super TEV protease | Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent. |
| 171928 | pET28-AviTag-DogCatcher-MBP | Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA. |
| 171929 | pDEST14-Cys-DogCatcher | Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin. |
| 171930 | pJ404-DogCatcher-sfGFP | Express DogCatcher-superfolder GFP protein in bacterial cytoplasm |