Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5.
Terzi EM, Sviderskiy VO, Alvarez SW, Whiten GC, Possemato R
Sci Adv. 2021 May 26;7(22). pii: 7/22/eabg4302. doi: 10.1126/sciadv.abg4302. Print 2021 May.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169880 | pCW57.1 ISCU D71A | Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative) |
| 169882 | pLKO.1P shTFRC | Suppress TRFC |
| 169884 | pLKO.1P shACO1 | Suppress ACO1 |
| 169885 | pLentiCRISPR V2 sgACO1_1 | Disrupt ACO1 |
| 169886 | pLentiCRISPR V2 sgIREB2_2 | Disrupt IREB2 |
| 169889 | pCW57.1 blast OFF 3xflag IREB2 | Express 3x flag IREB2, suppressible by doxycycline addition |
| 169890 | pMXS-IRES-BLAST TFR1 | Express TFR1 open reading frame. Lacks IRE regulatory elements |
| 169892 | pLentiCRISPR V2 sgACO1_2 | Disrput ACO1 |
| 169893 | pLentiCRISPR V2 sgACO1_3 | Disrupt ACO1 |
| 169894 | pLentiCRISPR V2 sgIREB2_1 | Disrupt IREB2 |