Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle.
Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, Possemato R
Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169818 | pMXS-IRES-BLAST flag-CAD | Expresses C-terminal flag-tagged CAD in mammalian cells |
| 169820 | pMXS-IRES-BLAST flag-CAD S1406A | Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site |
| 169821 | pMXS-IRES-BLAST flag-CAD S1859A | Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site |
| 169822 | pMXS-IRES-BLAST flag-CAD S1859E | Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site |
| 169823 | pMXS-IRES-BLAST flag-CAD T456A S1406A | Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site |
| 169824 | pMXS-IRES-BLAST flag-CAD T456A S1859A | Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site |
| 169826 | pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A | Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site |
| 169827 | pMXS-IRES-BLAST flag-CAD R2187A | Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD |
| 169828 | pMXS-IRES-BLAST flag-CAD M1601E | Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain |
| 169830 | pMXS-IRES-BLAST flag-CAD H1734A H1741A | Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain |
| 169831 | pMXS-IRES-BLAST flag-CAD delta Loop1 | Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain |
| 169832 | pMXS-IRES-BLAST flag-CAD delta Loop2 | Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain |
| 169833 | pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2 | Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain |
| 169835 | pMXS-IRES-BLAST flag-CAD delta Allosteric Domain | Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker |
| 188117 | pMXS-IRES-BLAST Halo-flag-CAD | Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells |
| 188118 | pMXS-IRES-BLAST EGFP-flag-CAD | Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells |
| 188119 | pMXS-IRES-BLAST flag-CAD S1345A | Expresses C-terminal flag-tagged human CAD S1345A in mammalian cells |
| 188120 | pMXS-IRES-BLAST flag-CAD T1348A | Expresses C-terminal flag-tagged human CAD T1348A in mammalian cells |
| 188121 | pMXS-IRES-BLAST flag-CAD F1366A | Expresses C-terminal flag-tagged human CAD F1366A in mammalian cells |
| 188122 | pMXS-IRES-BLAST flag-CAD G1347A | Expresses C-terminal flag-tagged human CAD G1347A in mammalian cells |
| 188123 | pMXS-IRES-BLAST flag-CAD W1364A | Expresses C-terminal flag-tagged human CAD W1364A in mammalian cells |
| 188124 | pMXS-IRES-BLAST flag-CAD N1394A | Expresses C-terminal flag-tagged human CAD N1394A in mammalian cells |
| 188125 | pMXS-IRES-BLAST flag-CAD L1395A | Expresses C-terminal flag-tagged human CAD L1395A in mammalian cells |
| 188126 | pMXS-IRES-BLAST flag-CAD S1396A | Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells |
| 188127 | pMXS-IRES-BLAST flag-CAD G1412A | Expresses C-terminal flag-tagged human CAD G1412A in mammalian cells |
| 188128 | pMXS-IRES-BLAST flag-CAD G-Loop1 | Expresses C-terminal flag-tagged human CAD G-Loop1 in mammalian cells |
| 188129 | pMXS-IRES-BLAST flag-CAD G-Loop2 | Expresses C-terminal flag-tagged human CAD G-Loop2 in mammalian cells |
| 188130 | pMXS-IRES-BLAST flag-CAD hCPS1 AD | Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells |
| 188131 | pMXS-IRES-BLAST flag-CAD R1398A | Expresses C-terminal flag-tagged human CAD R1398A in mammalian cells |
| 188132 | pMXS-IRES-BLAST flag-CAD R1403A | Expresses C-terminal flag-tagged human CAD R1403A in mammalian cells |
| 188133 | pMXS-IRES-BLAST flag-CAD R1404A | Expresses C-terminal flag-tagged human CAD R1404A in mammalian cells |
| 188134 | pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A | Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells |
| 188135 | pMXS-IRES-BLAST flag-CAD R1403A R1404A | Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells |
| 188136 | pMXS-IRES-BLAST flag-CAD L1405A | Expresses C-terminal flag-tagged human CAD L1405A in mammalian cells |
| 188137 | pMXS-IRES-BLAST flag-CAD A1400E | Expresses C-terminal flag-tagged human CAD A1400E in mammalian cells |
| 188138 | pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405A | Expresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells |
| 188139 | pMXS-IRES-BLAST flag-CAD C1374S | Expresses C-terminal flag-tagged human CAD C1374S in mammalian cells |
| 188140 | pMXS-IRES-BLAST flag-CAD E1367A | Expresses C-terminal flag-tagged human CAD E1367A in mammalian cells |
| 188141 | pMXS-IRES-BLAST flag-CAD E1368A | Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells |
| 188142 | pMXS-IRES-BLAST flag-CAD E1373A | Expresses C-terminal flag-tagged human CAD E1373A in mammalian cells |
| 188143 | pMXS-IRES-BLAST flag-CAD E1367A E1368A | Expresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells |
| 188144 | pMXS-IRES-BLAST flag-CAD E1367A E1368A E1373A | Expresses C-terminal flag-tagged human CAD E1367A E1368A E1373A in mammalian cells |
| 188145 | pMXS-IRES-BLAST flag-CAD D1371A | Expresses C-terminal flag-tagged human CAD D1371A in mammalian cells |
| 188146 | pMXS-IRES-BLAST flag-CAD P1375A P1376A | Expresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells |
| 188147 | pMXS-IRES-BLAST flag-CAD 3E-3R | Expresses C-terminal flag-tagged human CAD 3E-3R in mammalian cells |
| 188148 | pMXS-IRES-BLAST flag-CAD 3R-3E | Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells |
| 188149 | pMXS-IRES-BLAST flag-CAD 3E@3R | Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells |
| 188150 | pMXS-IRES-BLAST flag-CAD hCPS1 TL | Expresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian cells |