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A genome-wide transcriptome and translatome analysis of Arabidopsis transposons identifies a unique and conserved genome expression strategy for Ty1/Copia retroelements.

Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. Epub 2017 Aug 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167119p35S_EVDwtPlant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.
167120p35S_EVDdiPlant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.
167121p35S_EVDm1Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.
167122p35S_GFP-GUSPlant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.
167123p35S_GFP-EVDinter-GUSPlant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

Antibodies from Article