Acute depletion of METTL3 implicates N (6)-methyladenosine in alternative intron/exon inclusion in the nascent transcriptome.

Wei G, Almeida M, Pintacuda G, Coker H, Bowness JS, Ule J, Brockdorff N
Genome Res. 2021 Aug;31(8):1395-1408. doi: 10.1101/gr.271635.120. Epub 2021 Jun 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
165420	pSpCas9_U6_sgRNA_Mettl3C	sgRNA construct for targeting Mettl3 C-terminus
165421	pTargeting_Mettl3C_FKBP-V	Template for knockin FBKP-V into Mettl3 C-terminus