An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat.
Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA
Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
165418 | pGGG Wheat GUS marker | Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter |
165419 | pSOUP | The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium |