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An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat.

Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA
Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
165418pGGG Wheat GUS markerWheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
165419pSOUPThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Antibodies from Article