Epigenetic reprogramming of a distal developmental enhancer cluster drives SOX2 overexpression in breast and lung adenocarcinoma.
Abatti LE, Lado-Fernandez P, Huynh L, Collado M, Hoffman MM, Mitchell JA
Nucleic Acids Res. 2023 Sep 22:gkad734. doi: 10.1093/nar/gkad734.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163701 | AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP | Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide. |
| 163747 | gRNA_CloningVector_tagBFP | Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection. |
| 163748 | gRNA_CloningVector_miRFP670 | Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection. |
| 163751 | SOX2-P2A-tagBFP-HDR | HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide. |
| 163752 | gRNA_SOX2_HDR | gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells. |
| 163753 | gRNA_SRR124.5_tagBFP | gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells. |
| 163754 | gRNA_SRR134.3_miRFP670 | gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells. |
| 187222 | CMV-NFIB-T2A-miRFP670 | Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter. |
| 187223 | CMV-hCas9-T2A-miRFP670 | Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter. |
| 187224 | CMV-hCas9-T2A-mStrawberry | Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter. |
| 187375 | CMV-SOX2-T2A-miRFP670 | Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter. |