Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay.
Lyu P, Yoo KW, Yadav MK, Atala A, Aartsma-Rus A, Putten MV, Duan D, Lu B
PLoS One. 2020 Sep 24;15(9):e0239468. doi: 10.1371/journal.pone.0239468. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138353 | pSin-EF2-DMD53-GFP | Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences |