Modular transient nanoclustering of activated beta2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies.
Gormal RS, Padmanabhan P, Kasula R, Bademosi AT, Coakley S, Giacomotto J, Blum A, Joensuu M, Wallis TP, Lo HP, Budnar S, Rae J, Ferguson C, Bastiani M, Thomas WG, Pardon E, Steyaert J, Yap AS, Goodhill GJ, Hilliard MA, Parton RG, Meunier FA
Proc Natl Acad Sci U S A. 2020 Nov 19. pii: 2007443117. doi: 10.1073/pnas.2007443117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162876 | pmEos2-N1 GBP (GBP-mEos2) | Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy |
| 162877 | pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2) | Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM |
| 162878 | pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1) | Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM |
| 162879 | pmCherry-N1 GBP (GBP-mCherry) | Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry |
| 162880 | pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1) | Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP |