The phospholipid PI(3,4)P2 is an apical identity determinant.
Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM
Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161990 | GFP-2xPH-TAPP1 WT | Expresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP |
| 161991 | GFP-2xPH-TAPP1 R211L mutant | Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP |
| 161992 | pQCXIH-tag RFPt- Rab11a WT | Retroviral expression vector containing Rab11a tagged with RFP |
| 161993 | mCherry-C1 SNX9 RYK mutant R286A Y287A K288A | Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag |
| 161994 | mCherry-C1 SNX9 KR mutant K267N R327N | Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag |
| 161995 | pmCherry-C1-INPP4B WT | Mammalian expression vector containing INPP4B. Also contains mCherry tag |
| 161996 | pmCherry-C1-INPP4B C842A mutant | Mammalian expression vector containing a phosphatase dead mutant of INPP4B (C842A). Also contains mCherry tag |
| 161997 | pRevTRE SGFP2 Inpp5E WT shRNA resistant | Tet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag. |
| 161998 | INPP5D-eGFP WT | Mammalian expression vector, containing a GFP tagged version of INPP5D |
| 161999 | INPP5D-eGFP D673G mutant | Mammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G) |
| 162000 | pLEX303 GFP-PIK3C2A | Lentiviral expression vector containing PIK3C2A tagged with GFP |
| 162001 | pLEX303 GFP-PIK3C2B | Lentiviral expression vector containing PIK3C2B tagged with GFP |
| 162003 | pLKO.1 Hygro shRNA INPP4B KD2 | INPP4B shRNA |
| 162004 | pLKO.1 Puro shRNA INPP5E KD1 | INPP5E shRNA |
| 162005 | pLKO.1 Puro shRNA INPP5E KD2 | INPP5E shRNA |
| 162006 | pLKO.1 Puro shRNA PIK3C2A KD5 | PIK3C2alpha shRNA |
| 162008 | pLKO.1 Puro shRNA PIK3C2G KD1 | PIK3C2gamma shRNA |
| 162009 | pLKO.1 Puro shRNA PTEN KD2 | PTEN shRNA |
| 162010 | pLKO.1 Puro shRNA PTEN KD4 | PTEN shRNA |
| 162011 | pLKO.1 Puro shRNA Scramble | Lentiviral negative control vector containing scramble shRNA |
| 162012 | pLKO.1 Puro shRNA SHIP1 KD1 | SHIP1 shRNA |
| 162013 | pLKO.1 Puro shRNA SHIP1 KD2 | SHIP1 shRNA |
| 162014 | pLKO.1 Puro shRNA SNX9 KD1 | SNX9 shRNA |
| 162015 | pLKO.1 Puro shRNA SNX9 KD2 | SNX9 shRNA |