INPP5D-eGFP D673G mutant
(Plasmid
#161999)
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PurposeMammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneeGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINPP5D
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Alt nameSHIP1
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SpeciesH. sapiens (human)
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MutationD673G
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Entrez GeneINPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)
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Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
INPP5D-eGFP D673G mutant was a gift from David Bryant (Addgene plasmid # 161999 ; http://n2t.net/addgene:161999 ; RRID:Addgene_161999) -
For your References section:
The phospholipid PI(3,4)P2 is an apical identity determinant. Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM. Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. 10.1038/s41467-018-07464-8 PubMed 30487552