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Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System.

Pullmann P, Weissenborn MJ
ACS Synth Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160911pPAP004E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris
160912pAGM9121_pAOX1Alcohol oxidase 1 (AOX1) promoter module for episomal P. pastoris promoter shuffling
160913pAGM9121_pFDH1Formate dehydrogenase (FDH1) promoter module for episomal P. pastoris promoter shuffling
160914pAGM9121_pFLD1Formaldehyde dehydrogenase (FLD1) promoter module for episomal P. pastoris promoter shuffling
160915pAGM9121_pPMP20Peroxisomal glutathione oxidase (PMP20) promoter module for episomal P. pastoris promoter shuffling
160916pAGM9121_pDAS1Dihydroxyacetone synthase 1 (DAS1) promoter module for episomal P. pastoris promoter shuffling
160917pAGM9121_pDAS2Dihydroxyacetone synthase 2 (DAS2) promoter module for episomal P. pastoris promoter shuffling
160918pAGM9121_pCAT1Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling
160919pAGM9121_pALD4Mitochondrial aldehyde dehydrogenase (ALD4) promoter module for episomal P. pastoris promoter shuffling
160920pAGM9121_pDHASDihydroxyacetone synthase (DHAS) promoter module for episomal P. pastoris promoter shuffling
160921pAGM9121_pFMDFormate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling
160922pAGM9121_pMOXMethanol oxidase (MOX) promoter module for episomal P. pastoris promoter shuffling

Antibodies from Article