pPAP004
(Plasmid
#160911)
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PurposeE.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPAP001
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacZ selection cassette
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SpeciesPichia pastoris
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPAP004 was a gift from Sylvestre Marillonnet (Addgene plasmid # 160911 ; http://n2t.net/addgene:160911 ; RRID:Addgene_160911) -
For your References section:
Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System. Pullmann P, Weissenborn MJ. ACS Synth Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641. 10.1021/acssynbio.0c00641 PubMed 34075757