ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.
Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BS
Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140155 | YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V) | Expresses a regulatable secretory cargo for yeast at the TRP1 locus |
| 140156 | YIplac128-pOst1-ESCargo(Crimson) | Expresses a regulatable secretory cargo for yeast at the LEU2 locus |
| 140157 | YIplac204-pOst1-ESCargo(Orange) | Expresses a regulatable secretory cargo for yeast at the TRP1 locus |
| 140158 | YIplac128-pOst1-ESCargo(Orange) | Expresses a regulatable secretory cargo for yeast at the LEU2 locus |
| 140159 | pEF-pIgH-ESCargo* | Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells |
| 140160 | pEF-pIgH-ESCargo(FTV) | Expresses a regulatable secretory cargo for mammalian cells |
| 140161 | pEF-pIgH-ESCargo(FTV,Orange) | Expresses a regulatable secretory cargo for mammalian cells |
| 140162 | pEF-pIgH-ESCargo(FTV,Crimson) | Expresses a regulatable secretory cargo for mammalian cells |
| 140163 | pcDNA-GalNAc-T2-GFP-ESCargo(FTV) | Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker |
| 140164 | pEF-GalNAc-T2-msGFP2 | Expresses the Golgi marker GalnacT2-msGFP2 |
| 140165 | pUASt-ssBiP-ESCargo* | Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster |
| 140166 | pUASt-ssBiP-ESCargo | Expresses a regulatable secretory cargo for Drosophila melanogaster |
| 140167 | NCVB-ssGrl1-ESCargo* | Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila |
| 140168 | ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V) | Expresses a regulatable secretory cargo for Tetrahymena thermophila |
| 140169 | pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V) | Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana |
| 140170 | pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V) | Expresses a regulatable secretory cargo for Arabidopsis thaliana |
| 160905 | pEF-ManII-GFP | Expresses the Golgi marker ManII-GFP in mammalian cells |