NAADP-regulated two-pore channels drive phagocytosis through endo-lysosomal Ca(2+) nanodomains, calcineurin and dynamin.

Davis LC, Morgan AJ, Galione A
EMBO J. 2020 Jul 15;39(14):e104058. doi: 10.15252/embj.2019104058. Epub 2020 Jun 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
207147	FKBP12-CB2mutx2-mTagBFP2-HAx2	Tandem mutant calcium-buffer with rapalog dimerizer
207146	FKBP12-CB2x2-mTagBFP2-HAx2	Tandem calcium-buffer with rapalog dimerizer
207145	LAMP1-mCherry-FRB*	Lysosome-targeted rapalog dimerizer
207144	TRPML1-G-GECO1.2-ERES	Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal
207143	TPC1-G-GECO1.2	Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel
207142	TPC2-D276K-G-GECO1.2	Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel
207141	TPC2-G-GECO1.2	Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel